B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains; a 21 aa transmembrane segment; and a 2 aa cytoplasmic tail. It is widely expressed; including in kidney; liver; lung; pancreas; placenta; prostate; spleen; testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation; proliferation; cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages; plays an important role; together with regulatory T-cells (Treg); in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.
Product Name:
Human B7-H4/VTCN1 Recombinant Protein (RPES4341)
Product Code:
RPES4341
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
B7S1, B7x, Vtcn1, B7h.5, B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1VCTN1, B7XPRO1291, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1
Accession:
Q7Z7D3
Sequence:
Phe29-Ala258
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human B7-H4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a Fc tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
52.5 kDa
AP Mol Mass:
70-95 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
