Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.
Product Name:
Human B4GALT1/GGTB2 Recombinant Protein (RPES1700)
Product Code:
RPES1700
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
B4GAL-T1,beta4Gal-T1,CDG2D,GGTB2,GT1,GTB
Accession:
NP_001488.2
Sequence:
Gly 44-Ser 398
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human B4GALT1 extracellular domain (NP_001488.2) (Gly 44-Ser 398) was fused with a polyhistidine tag at the N-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
41.5 kDa
AP Mol Mass:
45-55 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
