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Recombinant Human Argonaute-1/AGO1 Protein (RPCB2190)

SKU:
RPCB2190
Size:
50µg
Tag:
N-His
Reactivity:
Human
Expression Host:
Baculovirus-Insect Cells
€699
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Description

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Product Name:Recombinant Human Argonaute-1/AGO1 Protein
SKU:RPCB2190
Size:50µg
Tag:N-His
Reactivity:Human
Expression Host:Baculovirus-Insect Cells
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human Argonaute-1/AGO1 Protein , tested reactivity in Baculovirus-Insect Cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 1.0 EU/μg of the protein by LAL method.
Purity:>85% as determined by SDS-PAGE
Formulation:Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0.
Gene ID:26523

Protein argonaute-1, also known as eukaryotic translation initiation factor 2C 1, EIF2C1, and AGO1, is a member of the argonaute family and ago subfamily. Protein argonaute-1 in humans is encoded by the EIF2C1 gene. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4. This genomic region is frequently lost in human cancers such as Wilms tumors, neuroblastoma, and carcinomas of the breast, liver, and colon. The human EIF2C1 gene is ubiquitously expressed at low to medium levels. Differential polyadenylation and splicing result in a complex transcriptional pattern.EIF2C1 protein contains onePAZ domain and onePiwi domain. It is required for RNA-mediated gene silencing (RNAi) and transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). EIF2C1 binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:Q9UL18
Product ImageRecombinant Human Argonaute-1/AGO1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100 kDa.

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