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Human AMPK (G1/B1/A2) Heterotrimer Recombinant Protein (Active) (RPES0595)

SKU:
RPES0595
Product Type:
Recombinant Protein
Species:
Human
Research Area:
Metabolism
€1,949

Description

system_update_altDatasheet
Product Name:AMPK (G1/B1/A2) Recombinant Protein (RPES0595)
Product Code:RPES0595
Size:50 µg
Species:Human
Expressed Host:Baculovirus-Insect Cells
Synonyms:AMPK,AMPKa1
Accession:P54619
Sequence:Met 1-Pro 331
Fusion tag:
Activity:The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Endotoxin:<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270); constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Purity:> 94 % as determined by reducing SDS-PAGE.
Mol Mass:158 (38+30+90) kDa
AP Mol Mass:40; 42 & 95 kDa
Formulation:Supplied as sterile 50mM Tris; 200mM NaCl; 1mM EDTA; 1mM DTT; 0.5mM PMSF; 10% gly; 1mM GSH; pH 7.4
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

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