Adenylate kinase isoenzyme 4; mitochondrial; also known as ATP-AMP transphosphorylase; Adenylate kinase 3-like; AK4 and AK3L1; is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases control the adenine and guanine nucleotide compositions in a cell by allowing for the transfer of phosphate groups among these components. In vertebrates, five isozymes of adenylate kinase have been discovered. Isozymal variants of these enzymes are tissue-specific and developmentally controlled. AK4/AK3L1 is a kinase that participates in the irreversible transfer of the final phosphate group from ATP to AMP. It may also be involved in GTP production. Although adenylate kinase 4 (AK4/AK3L1) is a unique individual that has no enzymatic activity in the adenylate kinase (AK) family, it shows high sequence similarity with other AKs. It's unclear what physiological function AK4 serves and why it is enzymatically inactivated. It is also notable that the AK4 /AK3L1 variant retains the ability to bind nucleotides. It has glutamine instead of an arginine molecule in the active site. The enzymatically inactive AK4 is a stress-responsive protein that is necessary for cell survival and growth. AK4 / AK3L1 is likely to require interaction with the mitochondrial inner membrane protein ANT in order for it to provide protection to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously. Human AK4/AK3L1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human AK4/AK3L1 Recombinant Protein (RPES5161)
Product Code:
RPES5161
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
AK3,AK3L1,AK3L2,AK4
Accession:
P27144-1
Sequence:
Ala 2-Tyr 223
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human AK4 (P27144-1) (Ala 2-Tyr 223) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
25.3 kDa
AP Mol Mass:
27 kDa
Formulation:
Lyophilized from sterile 20mM Tris; 500mM NaCl; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
