Recombinant Human ACE2 Protein (His Tag) (RPES8544)
- SKU:
- RPES8544
- Size:
- 20μg
- Tag:
- C-His
- Reactivity:
- Human
- Expression Host:
- Baculovirus-Insect Cells
- Accession:
- NP_068576.1
Description
| Product Name: | Recombinant Human ACE2 Protein (His Tag) |
| SKU: | RPES8544 |
| Size: | 20μg |
| Tag: | C-His |
| Reactivity: | Human |
| Expression Host: | Baculovirus-Insect Cells |
| Accession: | NP_068576.1 |
| Sequence: | Met1-Ser740 |
| Calculated Molecular Weight: | 85.1 kDa |
| Synonyms: | ACE-Related Carboxypeptidase, ACE2, ACEH, Angiotensin-Converting Enzyme 2, Angiotensin-Converting Enzyme Homolog, Metalloprotease MPROT15 |
| Endotoxin: | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity: | > 93 % as determined by reducing SDS-PAGE. |
Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder.
| Bio-activity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag)(PKSR030500) at 2μg/mL (100μL/well) can bind human ACE2 protein (PKSR030510), the EC50 of Recombinant Human ACE2 Protein (His Tag) (PKSR030510) is 10-35ng/mL. |
| Formulation: | Lyophilized from sterile 20 mM PBS, 300 mM NaCl, 10% glycerol, pH 7.0.Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the printed manual. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
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