The CD244 antigen; also known as 2B4; is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production; which is expressed on all NK cells; a subpopulation of T cells; monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor; human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity; induce secretion of IFN-γ and matrix metalloproteinases (MMPs); as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2; CD48; CD58; CD84; Ly-9; and SLAM; 2B4 (CD244) is expressed on all human NK cells; a subpopulation of T cells; basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity; induces secretion of IFN-gamma and matrix metalloproteinases; and NK cell invasiveness.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human 2B4/CD244 Recombinant Protein (RPES2722)
Product Code:
RPES2722
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NAIL, NKR2B4, h2B4, SLAM family member 4, SLAMF4, CD244, 2B4
Accession:
NP_057466.1
Sequence:
Met 1-Arg 221
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human 2B4 at 2 µg/ml (100 ?l/well) can bind human CD48. The EC50 of human CD48 is 0.39 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Arg 221) of human 2B4 (NP_057466.1) was expressed; with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
23.8 kDa
AP Mol Mass:
45-50 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
