β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. β-galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose.
Product Name:
E.coli Beta-galactosidase Recombinant Protein (RPES3494)
Product Code:
RPES3494
Size:
10µg
Species:
E.coli
Expressed Host:
E.coli
Synonyms:
Beta-galactosidase, Beta-gal, Lactase, lacZ
Accession:
P00722
Sequence:
Met1-Lys1024(12-41AA deletion)
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant E.coli Beta-galactosidase(M443L, C500S) is produced by our E.coli expression system and the target gene encoding Met1-Lys1024(12-41AA deletion) is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
112.9 kDa
AP Mol Mass:
115 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
