The RDH5 Polyclonal Antibody (CAB8055) is a valuable tool for researchers studying retinol dehydrogenase 5 (RDH5), an essential enzyme involved in the visual cycle. This antibody, produced in rabbits, is highly specific and reacts with human samples, making it ideal for use in Western blot applications. By binding to the RDH5 protein, this antibody enables accurate detection and analysis in a variety of cell types, facilitating research in the fields of ophthalmology and vision biology.RDH5 is a key player in the process of converting all-trans-retinol to 11-cis-retinal, a crucial step in the regeneration of visual pigments in the retina.
Mutations in the RDH5 gene have been associated with various forms of retinal degeneration and visual impairments. Understanding the function and regulation of RDH5 is essential for developing targeted therapeutic interventions for these debilitating conditions.This RDH5 Polyclonal Antibody offers researchers a reliable tool for investigating the role of RDH5 in normal vision processes and in the pathogenesis of retinal diseases. Its high specificity and sensitivity make it a valuable asset in the quest to unravel the complexities of retinoid metabolism and to develop novel treatments for inherited retinal disorders.
Product Name:
RDH5 Rabbit Polyclonal Antibody
SKU:
CAB8055
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).
This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S1 (biogenesis of lysosomal organelles complex-1, subunit 1) gene.
Purification Method:
Affinity purification
Gene ID:
5959
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RDH5 antibody (CAB8055) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
