The RCOR1 Polyclonal Antibody (CAB12845) is a valuable tool for research involving the RCOR1 protein, also known as REST corepressor 1. This cell surface molecule is involved in multiple cellular processes, including gene regulation and neurodevelopment. The antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting.RCOR1 plays a critical role in epigenetic regulation and transcriptional repression, making it a key player in numerous biological processes. Its involvement in neural development and function also makes it a potential target for research in neurobiology and neurodegenerative diseases.
By binding to the RCOR1 protein, this antibody enables the detection and analysis of RCOR1 in various cell types, providing researchers with valuable insights into its functions and mechanisms of action. Understanding the role of RCOR1 is essential for advancing our knowledge of gene regulation, neurodevelopment, and disease pathways. This antibody offers researchers a reliable tool for studying RCOR1 in the context of various research areas, including neuroscience, developmental biology, and epigenetics.
Product Name:
CoREST/RCOR1 Rabbit Polyclonal Antibody
SKU:
CAB12845
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 256-485 of human CoREST/CoREST/RCOR1 (NP_055971.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
RCOR; COREST; CoREST/RCOR1
Positive Sample:
HeLa,Jurkat,Mouse testis,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
60kDa
This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor).
Purification Method:
Affinity purification
Gene ID:
23186
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CoREST/RCOR1 Rabbit pAb (CAB12845) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
