The RCN3 Polyclonal Antibody (PACO60312) is a research tool designed for investigating RCN3, a protein involved in various cellular processes. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By targeting the RCN3 protein, this antibody enables the detection and analysis of RCN3 in different cell types, making it suitable for studies in cell biology and molecular biology research.
RCN3, also known as reticulocalbin-3, plays a crucial role in calcium homeostasis and protein folding in the endoplasmic reticulum. Its involvement in these processes makes it a potential target for research in various diseases, including neurodegenerative disorders, cancer, and metabolic diseases. Understanding the function of RCN3 can provide insights into the mechanisms underlying these conditions and may lead to the development of novel therapeutic strategies.
IHC image of PACO60312 diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot. Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, Mouse heart tissue. All lanes: RCN3 antibody at 4.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 kDa. Observed band size: 38 kDa.
IHC image of PACO60312 diluted at 1:200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Synonyms:
Reticulocalbin-3 (EF-hand calcium-binding protein RLP49), RCN3
UniProt Protein Function:
RCN3: Belongs to the CREC family.Protein type: Secreted; Secreted, signal peptideChromosomal Location of Human Ortholog: 19q13.33Cellular Component: endoplasmic reticulum lumenMolecular Function: protein binding; calcium ion binding
