The RC3H1 Polyclonal Antibody (CAB15569) is a powerful tool for studying the RC3H1 protein, which is involved in post-transcriptional regulation of mRNA stability. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the RC3H1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component in molecular biology and genetic research.RC3H1, also known as Roquin-1, is a key player in RNA decay pathways, controlling the levels of target mRNAs and affecting gene expression.
Its role in RNA regulation has implications for various cellular processes, including immune response modulation and development. Research into RC3H1 can provide insights into diseases such as autoimmune disorders, inflammatory conditions, and cancer, where dysregulation of mRNA stability is a factor. Understanding the function of RC3H1 is crucial for advancing our knowledge of gene expression control and its impact on human health.
Product Name:
RC3H1 Rabbit Polyclonal Antibody
SKU:
CAB15569
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human RC3H1 (NP_742068.1).
This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
149041
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from SH-SY5Y cells, using RC3H1 Rabbit pAb (CAB15569) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
