The RBPJ Polyclonal Antibody (CAB5675) is a valuable tool for researchers studying the role of RBPJ, a key transcription factor in the Notch signaling pathway. This antibody, produced in rabbits, is highly specific to human samples and is optimized for use in Western blot applications. By binding to the RBPJ protein, this antibody allows for the detection and analysis of RBPJ expression in various cell types, making it essential for studies in developmental biology, cancer research, and stem cell biology.RBPJ, also known as recombination signal binding protein for immunoglobulin kappa J region, is a critical regulator of gene expression involved in cell fate decisions and development.
Its importance in controlling cell differentiation and proliferation makes it a target of interest in understanding diseases such as cancer, Alzheimer's, and developmental disorders. By investigating the function of RBPJ, researchers can gain insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets.
Product Name:
RBPJK Rabbit Polyclonal Antibody
SKU:
CAB5675
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RBPJKK (NP_976028.1).
The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.
Purification Method:
Affinity purification
Gene ID:
3516
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse liver, using RBPJKK Rabbit pAb (CAB5675) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
