The RBP3 Polyclonal Antibody (CAB6403) is a crucial tool for researchers investigating RBP3, also known as Retinol Binding Protein 3. RBP3 is a key player in the transport of retinol in the blood and plays a critical role in the visual cycle. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RBP3 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential tool for studies in ophthalmology, metabolism, and vision research.
The role of RBP3 in visual function and retinoid metabolism makes it a significant target for research into eye diseases, vitamin A deficiency, and metabolic disorders. Understanding the function and regulation of RBP3 is essential for developing interventions and treatments for conditions impacting vision and metabolism. The RBP3 Polyclonal Antibody provides researchers with a reliable and specific tool to further explore the functions and mechanisms of RBP3 in various biological contexts.
Product Name:
RBP3 Rabbit Polyclonal Antibody
SKU:
CAB6403
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-320 of human RBP3 (NP_002891.1).
Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.
Purification Method:
Affinity purification
Gene ID:
5949
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RBP3 antibody (CAB6403) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
