The RBFOX3 Polyclonal Antibody (CAB17261) is specifically designed for use in research involving the RBFOX3 protein, which is known to play a critical role in neuronal development and function. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.RBFOX3, also known as NeuN, is a neuron-specific RNA-binding protein that regulates alternative splicing in the brain, impacting neuronal maturation and synaptic function. Its expression is commonly used as a marker for mature neurons, making this antibody an essential tool for studying neurogenesis, neuronal differentiation, and neurodegenerative diseases such as Alzheimer's and Parkinson's.
By enabling the detection and analysis of RBFOX3 protein in various cell types, this antibody is ideal for neuroscience research and drug discovery targeting neuronal function. Understanding the role of RBFOX3 in brain development and neuronal plasticity is crucial for advancing our knowledge of neurological disorders and developing potential treatments.
Product Name:
NeuN Polyclonal Antibody
SKU:
CAB17261
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NeuN (NP_001076044.1).
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
146713
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NeuN Rabbit pAb (CAB17261) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
