The RBFOX3 Polyclonal Antibody (CAB15959) is a valuable tool for researchers studying the RBFOX3 protein, also known as NeuN, which is a marker of mature neurons in the central nervous system. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications.RBFOX3 is essential for neuronal development and function, playing a crucial role in regulating alternative splicing of RNA transcripts in neurons. Its expression is restricted to neurons, making it a useful marker for identifying and studying neuronal populations in the brain.
The RBFOX3 Polyclonal Antibody enables precise detection and analysis of RBFOX3 protein levels in various cell types, making it an ideal tool for neuroscience and neurobiology research.Research involving RBFOX3 has implications for understanding neurological disorders, neurodegenerative diseases, and brain injuries. By studying the expression and function of RBFOX3 using this antibody, researchers can gain valuable insights into neuronal development, synaptic plasticity, and neuronal survival, ultimately contributing to advancements in the field of neuroscience.
Product Name:
NeuN Rabbit Polyclonal Antibody
SKU:
CAB15959
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1).
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
146713
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251MG cells, using NeuN Rabbit pAb (CAB15959) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
