The RBFOX3 Polyclonal Antibody (CAB0951) is a valuable tool for researchers studying RBFOX3, a gene involved in neuronal development and function. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RBFOX3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for neurobiology and neurodegenerative disease research.
RBFOX3, also known as Neuronal RNA-binding protein HuC, plays a crucial role in regulating alternative splicing and mRNA translation in neurons. Dysregulation of RBFOX3 has been linked to neurodevelopmental disorders and neurodegenerative diseases such as Alzheimer's and Parkinson's. Investigating the function and expression of RBFOX3 is essential for understanding its involvement in these conditions and potentially identifying therapeutic targets for treatment.
Product Name:
[KO Validated] NeuN Rabbit Polyclonal Antibody
SKU:
CAB0951
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of NeuN (NP_001076044.1).
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
146713
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NeuN Rabbit pAb (CAB0951) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
