The RB1 Polyclonal Antibody (CAB11409) is a vital tool for researchers studying the RB1 gene, which plays a key role in cell cycle regulation and tumor suppression. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting RB1 protein in human samples, making it an essential tool for Western blot applications.RB1, also known as the retinoblastoma protein, is a tumor suppressor that acts as a gatekeeper for cell division, preventing uncontrolled proliferation and tumorigenesis. Dysregulation of RB1 is commonly observed in various cancers, making it a crucial target for cancer research and drug development.
By utilizing the RB1 Polyclonal Antibody, researchers can accurately detect and analyze RB1 protein levels in different cell types, aiding in the investigation of its role in cancer biology and potential therapeutic interventions.Overall, the RB1 Polyclonal Antibody is a valuable resource for researchers interested in exploring the functions and mechanisms of RB1 in cell cycle control and cancer development. Its reliability and versatility make it an ideal choice for immunology and oncology studies, providing valuable insights into the molecular pathways involved in tumorigenesis and potential targets for anti-cancer therapies.
Product Name:
RB Rabbit Polyclonal Antibody
SKU:
CAB11409
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human RB (NP_000312.2).
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RB antibody (CAB11409) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
