null

Rat Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit

SKU:
RTDL01115
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZCR1
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
INT4,Proto-oncogene Int-4 homolog
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit

The Rat Wingless-Type MMTV Integration Site Family Member 3 (Wnt3) ELISA Kit is an essential tool for accurate detection of Wnt3 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Wnt3 is a key signaling protein involved in the Wnt signaling pathway, playing a critical role in cell proliferation, differentiation, and tissue development.

Dysregulation of Wnt3 expression has been linked to various diseases, including cancer, developmental disorders, and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat Wnt3 ELISA kit is a reliable and efficient tool for researchers studying the role of Wnt3 in disease pathogenesis and therapeutic targeting.

Product Name:Rat Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit
Product Code:RTDL01115
Size:96 Assays
Target:WNT3
Synonyms:INT4, Proto-oncogene Int-4 homolog
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products