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Rat Viperin ELISA Kit

SKU:
RTDL01109
Product Type:
ELISA Kit
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Cig5,RSAD2,vig1,Radical S-Adenosyl Methionine Domain Containing 2,Viperin,Endoplasmic Reticulum-Associated,Interferon-Inducible,Cytomegalovirus-induced gene 5
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Viperin ELISA Kit

The Rat Viperin ELISA Kit is a powerful tool for researchers looking to study viperin levels in rat samples. This kit is designed for the accurate detection of viperin in serum, plasma, and cell culture supernatants with high sensitivity and specificity, ensuring reliable and reproducible results.Viperin is an important protein involved in the immune response to viral infections, playing a key role in antiviral defense mechanisms.

It is also involved in regulating inflammation and immune cell functions, making it a valuable biomarker for studying immune responses and developing potential therapeutics.By utilizing the Rat Viperin ELISA Kit, researchers can gain valuable insights into the role of viperin in various physiological and pathological conditions, advancing our understanding of viral infections and immune responses in rats.

Product Name:Rat Viperin ELISA Kit
Product Code:RTDL01109
Size:96 Assays
Target:Viperin
Synonyms:Cig5, RSAD2, vig1, Radical S-Adenosyl Methionine Domain Containing 2, Viperin,Endoplasmic Reticulum-Associated,Interferon-Inducible, Cytomegalovirus-induced gene 5
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)86-10093
EDTA plasma (n=5)87-10294
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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