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Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit

SKU:
RTDL01110
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F1LPV2
Sensitivity:
0.31ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
VLDL-R
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit

The Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit is a powerful tool for the quantification of VLDLR levels in rat samples. This kit is designed with high sensitivity and specificity to ensure accurate and reproducible results, making it an ideal choice for researchers studying lipid metabolism, cardiovascular diseases, and other related conditions.The VLDLR is a key receptor involved in the clearance of triglyceride-rich lipoproteins from circulation, playing a critical role in lipid homeostasis.

Dysregulation of VLDLR expression has been implicated in various metabolic disorders, making it a valuable biomarker for investigating these conditions and developing potential therapeutic strategies.With easy-to-follow protocols and reliable performance, the Rat VLDLR ELISA Kit from AssayGenie is a valuable asset for researchers looking to explore the role of VLDLR in physiological and pathological processes in rat models.

Product Name:Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit
Product Code:RTDL01110
Size:96 Assays
Target:VLDLR
Synonyms:VLDL-R
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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