Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
The Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit is a specialized assay designed for the precise quantification of VE-Cadherin levels in a variety of rat biological samples. VE-Cadherin, a vital cell adhesion molecule predominantly expressed in endothelial cells, plays a crucial role in maintaining vascular integrity, regulating endothelial cell function, and mediating vascular permeability. With its pivotal functions in endothelial cell adherens junctions and vascular homeostasis, VE-Cadherin serves as a key player in orchestrating vascular development, angiogenesis, and vascular function, making it an intriguing target for research in vascular biology, angiogenesis, and vascular-related diseases.
This ELISA kit offered by Assay Genie delivers exceptional sensitivity and specificity, ensuring accurate and reproducible measurement of VE-Cadherin levels. Manufactured under rigorous quality control standards, this kit provides reliable performance and ease of use, making it a preferred choice for researchers investigating the intricacies of VE-Cadherin in vascular biology and related pathophysiological processes. Unravel the significance of VE-Cadherin in vascular function and disease using this advanced ELISA kit, empowering cutting-edge research and therapeutic developments in cardiovascular research.
Product Name:
Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
SKU:
AEES00334
Target:
Rat VE-Cadherin (Vascular Endothelial Cadherin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VE-Cadherin. You can calculate the concentration of Rat VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
90-103
85-97
Average (%)
98
97
92
1:4
Range (%)
97-109
88-101
96-111
Average (%)
103
93
104
1:8
Range (%)
99-113
86-99
92-107
Average (%)
107
91
99
1:16
Range (%)
100-118
82-93
97-113
Average (%)
108
86
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.5
4.2
0.5
1.6
4.4
Standard deviation
0.03
0.07
0.22
0.03
0.07
0.18
C V (%)
6
4.67
5.24
6.0
4.38
4.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat VE-Cadherin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VE-Cadherin in samples. No significant cross-reactivity or interference between Rat VE-Cadherin and analogues was observed.
