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Rat Valosin Containing Protein (VCP) ELISA Kit

SKU:
RTDL01095
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P46462
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
IBMPFD,p97,TERA,Transitional Endoplasmic Reticulum ATPase,15S Mg(2+)-ATPase p97 subunit
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Valosin Containing Protein (VCP) ELISA Kit

The Rat Valosin-Containing Protein (VCP) ELISA Kit is specifically designed for the precise measurement of VCP levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity to ensure accurate and consistent results, making it an excellent choice for various research applications.VCP is a vital protein that plays a crucial role in protein quality control and degradation pathways. Dysregulation of VCP has been implicated in various diseases, including neurodegenerative disorders and cancer, highlighting its significance as a potential therapeutic target and biomarker for disease progression.

By utilizing the Rat VCP ELISA Kit, researchers can effectively study the role of VCP in disease pathogenesis and explore its therapeutic potential, ultimately advancing our understanding of VCP-related disorders and potentially developing novel treatment strategies.

Product Name:Rat Valosin Containing Protein (VCP) ELISA Kit
Product Code:RTDL01095
Size:96 Assays
Target:VCP
Synonyms:IBMPFD, p97, TERA, Transitional Endoplasmic Reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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