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Rat V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) ELISA Kit

SKU:
RTDL00901
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7TQN4
Sensitivity:
0.049ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NFKB3,p65,REL-A,Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3,Transcription Factor P65,Nuclear factor NF-kappa-B p65 subunit
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) ELISA Kit

The Rat v-Rel (Reticuloendotheliosis Viral Oncogene Homolog A Rela) ELISA Kit is a valuable tool for researchers looking to accurately detect levels of v-Rel in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.V-Rel is a viral oncogene homolog that plays a crucial role in cellular transformation and immune response regulation.

Its dysregulation has been implicated in various diseases, including cancer and inflammatory conditions, making it a key biomarker for studying these disorders and developing potential therapeutic interventions.Overall, the Rat v-Rel ELISA Kit offers researchers a reliable and efficient method for analyzing v-Rel levels in rat samples, advancing our understanding of its role in disease pathogenesis and progression.

Product Name:Rat V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) ELISA Kit
Product Code:RTDL00901
Size:96 Assays
Target:RELA
Synonyms:NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, Transcription Factor P65, Nuclear factor NF-kappa-B p65 subunit
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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