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Rat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

SKU:
RTDL00392
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12841
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Cfos,C-Fos,G0S7,Proto-oncogene c-Fos,G0/G1 Switch Regulatory Protein 7,Cellular oncogene fos
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

The Rat v-Fos/FBJ Murine Osteosarcoma Viral Oncogene Homolog FOS ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of FOS levels in rat samples. FOS is a proto-oncogene involved in cell proliferation, differentiation, and transformation, making it a key player in cancer research and therapy development.This ELISA kit offers reliable and reproducible results, making it suitable for a wide range of research applications in the field of oncology and molecular biology.

By measuring FOS levels in serum, plasma, or cell culture supernatants, researchers can gain insights into the mechanisms underlying tumor growth and progression, as well as potential therapeutic targets.Overall, the Rat v-Fos/FBJ Murine Osteosarcoma Viral Oncogene Homolog FOS ELISA Kit is an essential tool for studying the role of FOS in cancer biology and advancing our understanding of oncogenic signaling pathways.

Product Name:Rat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit
Product Code:RTDL00392
Size:96 Assays
Target:FOS
Synonyms:Cfos, C-Fos, G0S7, Proto-oncogene c-Fos, G0/G1 Switch Regulatory Protein 7, Cellular oncogene fos
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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