Rat Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit
The Rat Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit is a reliable tool for the precise measurement of UQCRC2 levels in rat serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for various research applications.UQCRC2 is a vital component of the electron transport chain, playing a crucial role in cellular respiration and energy production. Dysregulation of UQCRC2 has been linked to various diseases, including mitochondrial disorders and metabolic diseases, highlighting its significance as a potential biomarker for disease research and therapeutic development.
Trust the Rat UQCRC2 ELISA Kit to provide you with accurate and valuable insights into UQCRC2 levels, enabling you to advance your research and unlock new discoveries in the field of bioenergetics and mitochondrial biology. Elevate your research capabilities with this cutting-edge ELISA kit.
Product Name:
Rat Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit
Product Code:
RTDL01088
Size:
96 Assays
Target:
UQCRC2
Synonyms:
QCR2, UQCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
