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Rat TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit

SKU:
AEFI00852
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Reactivity:
Rat
€599
Frequently bought together:

Description

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Rat TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit

The Rat TWEAK (Tumour Necrosis Factor-Related Weak Inducer of Apoptosis) ELISA Kit is specifically designed for the quantitative measurement of TWEAK levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for researchers studying the role of TWEAK in various biological processes.TWEAK is a multifunctional cytokine that plays a critical role in regulating cell growth, survival, and apoptosis. It is known to be involved in inflammation, tissue regeneration, and immune response modulation.

Dysregulation of TWEAK expression has been linked to various diseases, including cancer, autoimmune disorders, and cardiovascular diseases, highlighting its importance as a potential therapeutic target.By using the Rat TWEAK ELISA Kit, researchers can gain valuable insights into the biological functions of TWEAK and its potential implications in disease pathogenesis. This kit offers a reliable and efficient method for quantifying TWEAK levels in rat samples, making it an essential tool for advancing research in the fields of immunology, oncology, and cardiovascular biology.

Product Name:Rat TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
Product Code:AEFI00852
Size:96T
Alias:TWEAK, TNFSF12, APO3 ligand, APO3LMGC20669, DR3LGTWEAKAPO3, DR3 ligand, MGC129581, TNF-related weak inducer of apoptosis, tumor necrosis factor, ligand superfamily, member 12, tumor necrosis factor ligand superfamily member 12
Detection method:Sandwich ELISA, Double Antibody
Application:
Sensitivity:< 9.375pg/ml
Range:15.625-1000pg/ml
Storage:2-8°C for 6 months
Note:For Research Use Only
Recovery: Matrices were spiked with Rat TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) and the recovery rates were calculated by comparing the measured value to the expected amount of Rat TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) in samples. Enquire for more information.
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Enquire for more information.
CV(%): Intra-Assay: CV<8%
Inter-Assay: CV<10%

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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