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Rat Tumor Protein p53 (TP53) ELISA Kit

SKU:
RTDL01053
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10361
Sensitivity:
28pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
p53,LFS1,TRP53,Li-Fraumeni Syndrome,Cellular tumor antigen p53,Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Tumor Protein p53 (TP53) ELISA Kit

The Rat Tumor Protein p53 (TP53) ELISA Kit is a powerful tool for measuring levels of the tumor suppressor protein p53 in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Tumor protein p53 (TP53) is a critical protein involved in regulating cell growth and preventing tumor formation.

Mutations in the TP53 gene are commonly found in various types of cancers, making it a valuable biomarker for studying cancer development and progression. By measuring TP53 levels in rat samples, researchers can gain valuable insights into the role of this protein in cancer biology and potentially develop new therapeutic strategies.

Product Name:Rat Tumor Protein p53 (TP53) ELISA Kit
Product Code:RTDL01053
Size:96 Assays
Target:TP53
Synonyms:p53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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