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Rat Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit (RTDL01061)

SKU:
RTDL01061
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
E9PU84
Sensitivity:
6.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD253,TNFSF10,APO2L,Apo2-L,TL2,TRAIL,Tumor Necrosis Factor Ligand Superfamily Member 10,Apo-2 Ligand
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649

Description

system_update_altDatasheet

Rat Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit

The Rat Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) ELISA Kit is a powerful tool for the quantitative measurement of TRAIL levels in rat serum, plasma, and tissue homogenates. This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TRAIL is a key protein involved in the induction of apoptosis, playing a critical role in immune surveillance and the regulation of cell death.

Dysregulation of TRAIL signaling has been implicated in various diseases, including cancer and autoimmune disorders, highlighting the importance of studying TRAIL levels for potential therapeutic interventions.With easy-to-follow protocols and reliable performance, the Rat TRAIL ELISA Kit from Assay Genie is a valuable asset for researchers investigating the role of TRAIL in disease pathogenesis and exploring novel treatment strategies.

Product Name:Rat Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit
Product Code:RTDL01061
Size:96 Assays
Target:TRAIL
Synonyms:CD253, TNFSF10, APO2L, Apo2-L, TL2, TRAIL, Tumor Necrosis Factor Ligand Superfamily Member 10, Apo-2 Ligand
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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