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Rat Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) ELISA Kit

SKU:
RTDL01044
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q4QQW2
Sensitivity:
5.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD40,CDW40,Bp50,P50,B-cell surface antigen CD40,CD40L receptor
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) ELISA Kit

The Rat Tumor Necrosis Factor Receptor Superfamily Member 5 (TNFRSF5) ELISA Kit is a valuable tool for the precise measurement of TNFRSF5 levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research purposes.TNFRSF5, also known as CD40, is a critical receptor involved in immune responses and inflammatory processes. It plays a vital role in regulating the activation of immune cells and promoting cytokine production.

Dysregulation of TNFRSF5 has been linked to various diseases, including autoimmune disorders and certain cancers, making it a key target for therapeutic development and understanding disease mechanisms.Overall, the Rat TNFRSF5 ELISA Kit provides a reliable and efficient method for quantifying TNFRSF5 levels in rat samples, facilitating research on immune responses, inflammation, and related diseases.

Product Name:Rat Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) ELISA Kit
Product Code:RTDL01044
Size:96 Assays
Target:TNFRSF5
Synonyms:CD40, CDW40, Bp50, P50, B-cell surface antigen CD40, CD40L receptor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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