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Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit

SKU:
RTDL01041
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06332
Sensitivity:
5.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
LT,LTA,TNF-B,TNFSF1,Tumor Necrosis Factor Ligand Superfamily Member 1,Lymphotoxin Alpha
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit

The Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit is a reliable and accurate tool designed for the quantitative detection of TNFb levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Tumor Necrosis Factor Beta (TNFb) is a key cytokine involved in inflammatory responses and immune regulation. It plays a crucial role in the pathogenesis of various diseases, including cancer, autoimmune disorders, and inflammatory conditions.

Studying TNFb levels can provide valuable insights into the mechanisms underlying these diseases and aid in the development of targeted therapies.With its user-friendly protocol and superior performance, the Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit is an indispensable tool for researchers seeking to investigate the role of TNFb in health and disease. Order yours today and take your research to the next level.

Product Name:Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit
Product Code:RTDL01041
Size:96 Assays
Target:TNFb
Synonyms:LT, LTA, TNF-B, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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