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Rat Transmembrane Protein 27 (TMEM27) ELISA Kit

SKU:
RTDL01038
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9ESG3
Sensitivity:
0.046ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NX17,Collectrin
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

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Rat Transmembrane Protein 27 (TMEM27) ELISA Kit

The Rat Transmembrane Protein 27 (TMEM27) ELISA Kit is specifically designed for the accurate detection of TMEM27 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TMEM27 is a transmembrane protein that plays a critical role in various cellular processes, including cell adhesion and signaling pathways. Dysregulation of TMEM27 has been linked to several diseases, making it an important biomarker for studying disease mechanisms and potential therapeutic interventions.

With the Rat TMEM27 ELISA Kit, researchers can confidently measure TMEM27 levels in biological samples, providing valuable insights into its role in disease progression and potential targets for drug development. Invest in this kit for accurate and efficient detection of TMEM27 levels in your research studies.

Product Name:Rat Transmembrane Protein 27 (TMEM27) ELISA Kit
Product Code:RTDL01038
Size:96 Assays
Target:TMEM27
Synonyms:NX17, Collectrin
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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