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Rat Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit

SKU:
RTDL01064
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9ESQ5
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TRPM4B,Melastatin-4,Calcium-activated non-selective cation channel 1,Long transient receptor potential channel 4
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Rat Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit

The Rat Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit is specifically designed for the accurate measurement of TRPM4 levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.TRPM4 is a vital ion channel involved in various physiological processes, including the regulation of calcium signaling and membrane potential.

Dysregulation of TRPM4 has been linked to conditions like hypertension, cardiac arrhythmias, and neurological disorders, highlighting its importance as a potential therapeutic target and diagnostic biomarker.With this TRPM4 ELISA Kit, researchers can confidently investigate the role of TRPM4 in disease pathogenesis and explore new avenues for intervention strategies. Trust in the reliability and accuracy of this kit for your research needs.

Product Name:Rat Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit
Product Code:RTDL01064
Size:96 Assays
Target:TRPM4
Synonyms:TRPM4B, Melastatin-4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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