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Rat Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

SKU:
RTDL01019
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07257
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B2,TGF-Beta2,G-TSF,LAP,Cetermin,Polyergin,Latency-associated peptide,BSC-1 cell growth inhibitor,Glioblastoma-derived T-cell suppressor factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

The Rat Transforming Growth Factor Beta 2 (TGFB2) ELISA Kit is specially designed for the accurate detection of TGFB2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TGFB2 is a key protein involved in regulating cell growth, differentiation, and immune response. It plays a crucial role in various biological processes, including development, wound healing, and tumorigenesis.

By accurately measuring TGFB2 levels, researchers can gain valuable insights into its role in disease pathogenesis and potential therapeutic targets.With its reliable performance and broad applicability, the Rat TGFB2 ELISA Kit is an essential tool for studying the complex biology of TGFB2 and its implications in various disease processes. Unlock the potential of TGFB2 research with this cutting-edge ELISA kit.

Product Name:Rat Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
Product Code:RTDL01019
Size:96 Assays
Target:TGFb2
Synonyms:TGF-B2, TGF-Beta2, G-TSF, LAP, Cetermin, Polyergin, Latency-associated peptide, BSC-1 cell growth inhibitor, Glioblastoma-derived T-cell suppressor factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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