The Rat TP53 (Tumor Protein 53) CLIA Kit is a cutting-edge assay meticulously crafted to quantitatively measure the levels of Tumor Protein 53 in rat biological samples.
Tumor Protein 53, a pivotal tumor suppressor gene, is crucial in governing cellular processes such as DNA repair, cell cycle regulation, and apoptosis. Its role in maintaining genomic stability, preventing tumorigenesis, and orchestrating cellular responses to stressors underscores its significance in cancer research, molecular medicine, and cell biology studies. By offering a precise and reliable method for quantifying TP53 levels, this CLIA Kit empowers researchers and clinicians to delve into the intricate mechanisms of tumor development, the molecular pathways associated with cancer progression, and the potential therapeutic interventions targeting TP53 pathways. The quantitative assessment of TP53 expression facilitated by this kit enables detailed investigations into the impact of TP53 dysregulation on cancer susceptibility and disease progression. With unparalleled sensitivity and specificity, the Rat TP53 CLIA Kit ensures accurate and reproducible results, delivering robust performance and high-quality data for comprehensive research and clinical applications. Manufactured under stringent quality control measures, this kit guarantees exceptional performance and user-friendly operation, making it an ideal choice for cutting-edge research endeavors and investigative studies in oncology and molecular biology.
Product Name:
Rat TP53 (Tumor Protein 53) CLIA Kit
SKU:
AEES00731
Target:
Rat TP53 (Tumor Protein 53)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TP53. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TP53. You can calculate the concentration of Rat TP53 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
95-107
93-107
Average (%)
95
101
100
1:4
Range (%)
98-112
103-117
100-114
Average (%)
105
108
106
1:8
Range (%)
98-114
102-117
87-101
Average (%)
105
109
93
1:16
Range (%)
102-115
85-100
101-116
Average (%)
108
91
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.57
129.74
409.47
56.2
135.66
428.0
Standard deviation
5.86
12.2
44.71
6.37
14.57
37.58
C V (%)
10.74
9.4
10.92
11.33
10.74
8.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TP53 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TP53 in samples. No significant cross-reactivity or interference between Rat TP53 and analogues was observed.
