Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit
The Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily Member 1A) CLIA Kit is an innovative assay designed for the precise measurement of TNFRSF1A levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.TNFRSF1A is a key receptor involved in the TNF signaling pathway, playing a crucial role in inflammation, cell survival, and immune response.
Dysregulation of TNFRSF1A has been implicated in numerous diseases, including autoimmune disorders, inflammatory conditions, and cancer. Therefore, monitoring TNFRSF1A levels can provide valuable insights into disease pathogenesis and aid in the development of targeted therapies.Overall, the Rat TNFRSF1A CLIA Kit is a valuable tool for researchers studying TNFRSF1A biology and its role in disease progression.
Product Name:
Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit
SKU:
RTES00603
Target:
Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNFRSF1A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNFRSF1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNFRSF1A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNFRSF1A. You can calculate the concentration of Rat TNFRSF1A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
99-112
94-107
Average (%)
107
105
100
1:4
Range (%)
86-103
98-114
90-105
Average (%)
94
104
98
1:8
Range (%)
97-111
96-109
96-111
Average (%)
105
103
103
1:16
Range (%)
96-111
91-103
92-106
Average (%)
104
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
106
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.61
67.85
190.04
23.74
64.1
182.27
Standard deviation
2.16
7.71
21.34
2.4
4.79
16.99
C V (%)
8.43
11.36
11.23
10.11
7.47
9.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TNFRSF1A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNFRSF1A in samples. No significant cross-reactivity or interference between Rat TNFRSF1A and analogues was observed.
