The Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of TNF-α levels in various rat biological samples.
TNF-α is a key pro-inflammatory cytokine with diverse roles in immune regulation, inflammation, cell proliferation, differentiation, and apoptosis. It plays a crucial role in the body's immune response by initiating inflammation and has been associated with various inflammatory diseases, autoimmune conditions, and infections. Accurate quantification of TNF-α using this ELISA kit is vital for understanding its impact on disease mechanisms, immune responses, and inflammatory cascades. Researchers can utilize the data generated by this kit to explore the role of TNF-α in various physiological and pathological conditions, paving the way for the development of targeted therapeutic strategies aimed at modulating TNF-α levels. With its exceptional sensitivity and specificity, the Rat TNF-α ELISA Kit from Assay Genie ensures dependable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an ideal choice for researchers conducting studies in the fields of immunology, inflammation, and disease research.
Product Name:
Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
SKU:
AEES00516
Target:
Rat TNF-α (Tumor Necrosis Factor Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-α. You can calculate the concentration of Rat TNF-α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
90-103
91-100
Average (%)
100
96
94
1:4
Range (%)
88-102
94-107
98-107
Average (%)
96
100
101
1:8
Range (%)
85-101
90-106
92-108
Average (%)
93
98
100
1:16
Range (%)
96-107
98-105
93-102
Average (%)
101
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
102-110
105
Cell culture media (n=5)
98-104
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.74
109.13
414.25
49.5
115.5
436.41
Standard deviation
2.3
5.13
16.78
2.53
5.51
23.48
C V (%)
5.38
4.7
4.05
5.11
4.77
5.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TNF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNF-α in samples. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed.
