The Rat Thrombin (TM) ELISA Kit is a specialized assay tool designed for the precise quantification of Thrombin levels in rat biological samples. Thrombin, also known as coagulation Factor II, is a pivotal serine protease that plays a crucial role in the coagulation cascade, fibrinolysis, and other physiological processes. Thrombin is a multifunctional enzyme involved in blood clotting, platelet aggregation, and inflammation. It catalyzes the conversion of fibrinogen into fibrin, leading to the formation of blood clots essential for wound healing and hemostasis. Additionally, Thrombin modulates various cellular responses through protease-activated receptors, influencing vascular permeability, inflammation, and cell proliferation. By accurately measuring Thrombin levels in rat samples, researchers can gain insights into the coagulation pathway, thrombotic disorders, cardiovascular diseases, and inflammatory responses.
The quantitative assessment of Thrombin using this ELISA kit enables the investigation of Thrombin's role in physiological and pathological conditions related to blood coagulation and vascular health. The Rat Thrombin ELISA Kit provided by Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance, making it a valuable tool for research studies focusing on Thrombin function, coagulation disorders, and related pathological conditions in rat models.
Product Name:
Rat TM (Thrombin) ELISA Kit
SKU:
AEES00384
Target:
Rat TM (Thrombin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TM. You can calculate the concentration of Rat TM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
89-104
93-111
Average (%)
92
95
101
1:4
Range (%)
91-105
88-100
82-95
Average (%)
97
93
88
1:8
Range (%)
86-100
81-94
86-98
Average (%)
93
87
92
1:16
Range (%)
88-98
85-96
86-97
Average (%)
93
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.35
3.58
0.47
1.44
3.5
Standard deviation
0.03
0.06
0.17
0.03
0.07
0.17
C V (%)
5.77
4.44
4.75
6.38
4.86
4.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TM in samples. No significant cross-reactivity or interference between Rat TM and analogues was observed.
