The Rat TLR4 (Toll-Like Receptor 4) ELISA Kit is specifically designed for the accurate measurement of TLR4 levels in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, suitable for a variety of research applications.TLR4 is a key receptor involved in the immune response, recognizing pathogen-associated molecular patterns and initiating the inflammatory cascade. Dysregulation of TLR4 signaling has been implicated in various diseases, including infectious diseases, autoimmune disorders, and chronic inflammatory conditions.
Therefore, this kit serves as a valuable tool for studying TLR4-mediated immune responses and developing potential therapeutic interventions.Don't miss out on the opportunity to investigate the role of TLR4 in rat models with the Rat TLR4 ELISA Kit. Order yours today from Assay Genie and take your research to the next level.
Product Name:
Rat TLR4 (Toll-Like Receptor 4) ELISA Kit
SKU:
RTES00829
Target:
Rat TLR4 (Toll-Like Receptor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TLR4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TLR4. You can calculate the concentration of Rat TLR4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
92-109
92-106
Average (%)
91
99
98
1:4
Range (%)
87-102
81-93
84-98
Average (%)
94
88
91
1:8
Range (%)
88-102
78-91
82-91
Average (%)
94
85
87
1:16
Range (%)
89-101
85-95
82-96
Average (%)
94
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-111
101
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.18
8.78
0.91
2.03
8.85
Standard deviation
0.06
0.13
0.29
0.05
0.11
0.31
C V (%)
6.32
5.96
3.3
5.49
5.42
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TLR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TLR4 in samples. No significant cross-reactivity or interference between Rat TLR4 and analogues was observed.
