The Rat TLR-2 (Toll-Like Receptor 2) CLIA Kit is a highly accurate and sensitive assay designed for the detection of TLR-2 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers excellent specificity, ensuring reliable and reproducible results that are essential for a variety of research applications.TLR-2 is a key protein involved in the recognition of pathogen-associated molecular patterns, playing a crucial role in the immune response to bacterial and fungal infections.
Abnormal TLR-2 activation has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Rat TLR-2 CLIA Kit provides researchers with a powerful tool for investigating the role of TLR-2 in immune responses and inflammatory diseases in rats, ultimately aiding in the advancement of scientific knowledge and the development of new treatment strategies.
Product Name:
Rat TLR-2 (Toll-Like Receptor 2) CLIA Kit
SKU:
RTES00524
Target:
Rat TLR-2 (Toll-Like Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TLR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TLR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TLR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TLR2. You can calculate the concentration of Rat TLR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
100-119
91-107
Average (%)
91
109
98
1:4
Range (%)
93-108
88-101
93-105
Average (%)
99
93
99
1:8
Range (%)
97-110
100-113
102-116
Average (%)
103
107
108
1:16
Range (%)
102-116
86-96
101-116
Average (%)
109
91
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
95-108
103
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.65
455.03
1985.78
236.47
439.91
1990.34
Standard deviation
21.28
33.99
222.01
28.4
44.83
212.37
C V (%)
9.82
7.47
11.18
12.01
10.19
10.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TLR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TLR2 in samples. No significant cross-reactivity or interference between Rat TLR2 and analogues was observed.
