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Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

SKU:
RTDL01029
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30121
Sensitivity:
0.60ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CSC-21K,TIMP Metallopeptidase Inhibitor 2
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

The Rat Tissue Inhibitors of Metalloproteinase 2 (TIMP2) ELISA Kit from Assay Genie is a reliable and accurate tool for measuring TIMP2 levels in rat tissue samples. This kit is designed with high sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.TIMP2 is an important protein that plays a critical role in regulating the activity of matrix metalloproteinases, which are enzymes involved in tissue remodeling and cell migration.

Dysregulation of TIMP2 has been implicated in various diseases, including cancer and cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With the Assay Genie Rat TIMP2 ELISA Kit, researchers can gain valuable insights into the role of TIMP2 in disease pathogenesis and progression, ultimately leading to the development of innovative treatment strategies.

Product Name:Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit
Product Code:RTDL01029
Size:96 Assays
Target:TIMP2
Synonyms:CSC-21K, TIMP Metallopeptidase Inhibitor 2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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