The Rat Thyroid Stimulating Hormone (TSH) ELISA Kit is specifically designed for the precise measurement of TSH levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it an invaluable tool for researchers studying thyroid function and related disorders in rats.Thyroid Stimulating Hormone (TSH) is a key regulator of thyroid gland activity, influencing the production and release of thyroid hormones. Abnormal TSH levels can indicate thyroid dysfunction, making it a vital biomarker for diagnosing and monitoring thyroid disorders in rats.
This ELISA kit provides a comprehensive solution for studying TSH dynamics and its role in maintaining thyroid health in experimental rat models.Overall, the Rat TSH ELISA Kit offers researchers a powerful tool for investigating thyroid function and pathology in rats, advancing our understanding of thyroid-related diseases and potential therapeutic interventions.
Product Name:
Rat Thyroid Stimulating Hormone (TSH) ELISA Kit
Product Code:
RTDL01068
Size:
96 Assays
Target:
TSH
Synonyms:
Thyrotropin
Detection Method:
Competitive
Range:
0.234-15ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
