Rat Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit
The Rat Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit from Assay Genie is a highly reliable and accurate tool for detecting TSHR levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this ELISA kit provides dependable and reproducible results, making it an ideal choice for a variety of research applications.The TSHR protein plays a crucial role in regulating thyroid function and metabolism, making it a key target for studying thyroid disorders and related conditions.
By accurately measuring TSHR levels, researchers can gain valuable insights into thyroid function and potential therapeutic strategies.Overall, the Rat Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit from Assay Genie is a valuable tool for investigating thyroid-related diseases and advancing our understanding of TSHR biology. With its high-quality performance and ease of use, this kit is an essential resource for researchers in the field of thyroid research.
Product Name:
Rat Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit
Product Code:
RTDL01069
Size:
96 Assays
Target:
TSHR
Synonyms:
LGR3, HTSHR-I, Thyrotropin Receptor, TSH Receptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
