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Rat Surfactant Associated Protein C (SPC) ELISA Kit

SKU:
RTDL00984
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11685
Sensitivity:
0.147ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SFTPC,PSP-C,SFTP2,SP-C,SP5,Pulmonary Surfactant Protein C,Pulmonary surfactant-associated proteolipid SPL(Val)
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates, Lung Lavage Fluid And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Surfactant Associated Protein C (SPC) ELISA Kit

The Rat Surfactant Associated Protein C (SP-C) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of SP-C levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.SP-C is an important protein found in surfactant, a substance that helps maintain the stability of the alveoli in the lungs and reduces surface tension, allowing for proper lung function.

Abnormalities in SP-C levels have been linked to lung diseases such as respiratory distress syndrome and pulmonary fibrosis, making it a valuable biomarker for studying these conditions and developing potential therapies.With its high performance and ease of use, the Rat Surfactant Associated Protein C (SP-C) ELISA Kit is a valuable tool for researchers studying lung biology, respiratory diseases, and surfactant function in rats.

Product Name:Rat Surfactant Associated Protein C (SPC) ELISA Kit
Product Code:RTDL00984
Size:96 Assays
Target:SPC
Synonyms:SFTPC, PSP-C, SFTP2, SP-C, SP5, Pulmonary Surfactant Protein C, Pulmonary surfactant-associated proteolipid SPL(Val)
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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