null

Rat Surfactant Associated Protein A (SPA) ELISA Kit

SKU:
RTDL00981
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08427
Sensitivity:
6.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
PRL,SFTPA,PSAP,PSPA,SFTP,SFTPA1,COLEC4,PSP-A,SFTP1,SFTPA1B,SP-A1,Collectin-4,Alveolar proteinosis protein,35 kDa pulmonary surfactant-associated protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Lung Lavage Fluid, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Surfactant Associated Protein A (SPA) ELISA Kit

The Rat Surfactant Associated Protein A (SPA) ELISA Kit is a highly sensitive and specific tool for the accurate measurement of surfactant associated protein A levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Surfactant associated protein A is an important component of lung surfactant, involved in lung function and immunity. Dysregulation of SPA has been implicated in various lung diseases, including respiratory distress syndrome and acute lung injury.

The Rat SPA ELISA Kit offers researchers a valuable tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Rat Surfactant Associated Protein A (SPA) ELISA Kit is a valuable resource for researchers interested in investigating the role of SPA in lung health and disease. Its high sensitivity and specificity make it a reliable choice for quantifying SPA levels in rat samples, leading to accurate and meaningful results.

Product Name:Rat Surfactant Associated Protein A (SPA) ELISA Kit
Product Code:RTDL00981
Size:96 Assays
Target:SPA
Synonyms:PRL, SFTPA, PSAP, PSPA, SFTP, SFTPA1, COLEC4, PSP-A, SFTP1, SFTPA1B, SP-A1, Collectin-4, Alveolar proteinosis protein, 35 kDa pulmonary surfactant-associated protein
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products