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Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit

SKU:
RTDL00974
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88583
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ATOD4,CIS3,Cish3,MGC71791,SOCS-3,SSI-3,SSI3,Cytokine-inducible SH2 protein 3,STAT-induced STAT inhibitor 3
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit

The Rat Suppressors of Cytokine Signaling 3 (SOCS3) ELISA Kit is a powerful tool for detecting and quantifying levels of SOCS3 in rat samples. With high sensitivity and specificity, this kit provides accurate and reliable results for researchers studying the regulation of cytokine signaling pathways.SOCS3 is a key protein involved in modulating immune responses and inflammatory processes. Its dysregulation has been implicated in various diseases, including autoimmune disorders and cancer.

By accurately measuring SOCS3 levels in rat serum, plasma, or tissue samples, researchers can gain valuable insights into the role of this protein in disease pathogenesis and potential therapeutic interventions.With its user-friendly protocol and robust performance, the Rat SOCS3 ELISA Kit is an indispensable tool for researchers investigating the intricate mechanisms of cytokine signaling and its impact on health and disease in rat models.

Product Name:Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Product Code:RTDL00974
Size:96 Assays
Target:SOCS3
Synonyms:ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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