Rat Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit
The Rat Superoxide Dismutase 2 (Mitochondrial SOD2) ELISA Kit is specifically designed for the accurate measurement of SOD2 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SOD2 is a critical enzyme that plays a key role in reducing oxidative stress and protecting cells from damage caused by free radicals in the mitochondria. Dysfunction of SOD2 has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.
Thus, measuring SOD2 levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of potential therapeutic interventions.Overall, the Rat Superoxide Dismutase 2 (Mitochondrial SOD2) ELISA Kit is an essential tool for researchers studying oxidative stress, mitochondria-related diseases, and potential antioxidant therapies in rats. Its reliability and accuracy make it a valuable asset in advancing scientific knowledge and improving healthcare outcomes.
Product Name:
Rat Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit
Product Code:
RTDL00976
Size:
96 Assays
Target:
SOD2
Synonyms:
IPO-B, MNSOD, Mn-SOD
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
