Rat Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit
The Rat SGPP2 (Sphingosine-1-Phosphate Phosphatase 2) ELISA Kit is a reliable tool for measuring levels of SGPP2 in rat biological samples such as serum, plasma, and tissue homogenates. This kit is designed with high sensitivity and specificity to ensure accurate and reproducible results for various research applications.SGPP2 is an essential enzyme involved in the regulation of sphingolipid metabolism, playing a critical role in cellular signaling pathways and lipid homeostasis. Dysregulation of SGPP2 has been implicated in various diseases, including cancer, inflammation, and autoimmune disorders, making it a valuable biomarker for studying these conditions and potential therapeutic targets.
With the Rat SGPP2 ELISA Kit, researchers can gain valuable insights into the role of SGPP2 in health and disease, aiding in the development of novel treatments and interventions. Trust in the accuracy and precision of this kit for your research needs in the field of sphingolipid metabolism and related disorders.
Product Name:
Rat Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit
Product Code:
RTDL00951
Size:
96 Assays
Target:
SGPP2
Synonyms:
SPP2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
