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Rat SPARC Like Protein 1 (SPARCL1) ELISA Kit

SKU:
RTDL00982
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P24054
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SC1,PIG33,Mast9,Hevin,High endothelial venule protein
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Rat SPARC Like Protein 1 (SPARCL1) ELISA Kit

The Rat SPARC-Like Protein 1 (SPARCL1) ELISA Kit is a reliable and sensitive assay designed to accurately measure SPARCL1 levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it suitable for a variety of research applications.SPARCL1 is a key protein involved in cell adhesion and tissue remodeling, playing a critical role in various biological processes. Its dysregulation has been linked to diseases such as cancer, fibrosis, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

With its high sensitivity and specificity, the Rat SPARCL1 ELISA Kit is an essential tool for researchers seeking to investigate the role of SPARCL1 in health and disease, paving the way for new insights and advancements in biomedical research.

Product Name:Rat SPARC Like Protein 1 (SPARCL1) ELISA Kit
Product Code:RTDL00982
Size:96 Assays
Target:SPARCL1
Synonyms:SC1, PIG33, Mast9, Hevin, High endothelial venule protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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