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Rat Solute Carrier Family 30 Member 4 (SLC30A4) ELISA Kit

SKU:
RTDL00961
Product Type:
ELISA Kit
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ZNT4,Zinc Transporter 4
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Rat Solute Carrier Family 30 Member 4 (SLC30A4) ELISA Kit

The Rat SLC30A4 ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of solute carrier family 30 member 4 (SLC30A4) levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of SLC30A4 in various biological processes.SLC30A4 is a key transporter protein involved in the regulation of zinc homeostasis in cells. Dysregulation of zinc levels has been linked to a variety of health conditions, including diabetes, Alzheimer's disease, and cancer.

By measuring SLC30A4 levels, researchers can gain valuable insights into the role of zinc transport in these diseases and potentially identify new therapeutic targets.Overall, the Rat SLC30A4 ELISA Kit is a valuable resource for researchers looking to investigate the function of SLC30A4 in health and disease, providing accurate and reliable data for a wide range of research applications.

Product Name:Rat Solute Carrier Family 30 Member 4 (SLC30A4) ELISA Kit
Product Code:RTDL00961
Size:96 Assays
Target:SLC30A4
Synonyms:ZNT4, Zinc Transporter 4
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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